Review



superblocktm buffer  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Thermo Fisher superblocktm buffer
    Superblocktm Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+tween+buffer/Tween+20+blocking+buffer%2C+1%25+in+PBS/pm42271423-86-12-14
    Average 95 stars, based on 1 article reviews
    superblocktm buffer - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Membrane:

    Article Title: Baccharis articulata aqueous extract exerts in vitro antifibrotic effect in hepatic stellate cells by attenuating collagen deposition and TGF-ß1 protein expression
    Article Snippet: After blocking, the individual primary horseradish peroxidase-conjugated antibodies (Santa Cruz Biotechnology, USA), PAI-1 (1:500 dilution), and TGF-β1 (1:500 dilution) were used to detect specific proteins. .. The membrane was subsequently washed thrice with PBS-Tween buffer, after which each blot was incubated with the corresponding secondary antibody for 1 h. Immunoreactive proteins were visualized by ECL detection using an iBright CL750 imaging system (Thermo Fisher Scientific, MA, USA). .. Expression levels were normalized against β-actin and quantified using the ImageJ software.

    Article Title: CP12 controls ribulose 1,5 bisphosphate recycling and carbon acquisition in Chlamydomonas reinhardtii
    Article Snippet: After one hour of blocking (5% fat free milk in PBS-Tween20 0.1% buffer), the membrane was incubated overnight with primary antibodies against EPYC1 (Agrisera, diluted 1:5000) or against PRK (antibodies kindly provided by Dr Pierre Crozet ( Boisset et al ., 2023 ), diluted 1:10000). .. After washing with PBS-Tween buffer, the membrane was incubated for one hour with a horseradish peroxidase (HRP) coupled anti-rabbit secondary antibodies (Invitrogen, diluted 1:10000). ..

    Article Title: Deficiency of skeletal muscle Agrin contributes to the pathogenesis of age-related sarcopenia in mice
    Article Snippet: Samples (50 μg protein) were resolved by SDS‒PAGE and transferred to a nitrocellulose membrane, which was incubated in 5% milk in phosphate buffered saline (PBS)-0.3% Tween 20(#A600560, Sangon Biotech) overnight at 4 °C and then with the following primary antibodies in 2% milk in PBS-Tween buffer: anti-MuSK (1:1000, sc-33204, Santa Cruz Biotechnology), anti-YAP (1:1000, #4912, Cell Signaling Technology), anti-YAP ser127 (1:500, #4911, Cell Signaling Technology), anti-Alpha Dystroglycan (1:500, ab106110, Abcam) and anti-GAPDH (1:2000, #437000, Thermo Scientific). .. After washing, the membrane was incubated with PBS-Tween buffer containing HRP-conjugated goat anti-mouse and anti-rabbit IgG from Pierce (1:2000, PI-32230 (anti-mouse), PI-32260 (anti-rabbit)). ..

    Article Title: Deficiency of skeletal muscle Agrin contributes to the pathogenesis of age-related sarcopenia in mice.
    Article Snippet: Samples (50 μg protein) were resolved by SDS‒PAGE and transferred to a nitrocellulose membrane, which was incubated in 5% milk in phosphate buffered saline (PBS)-0.3% Tween 20(#A600560, Sangon Biotech) overnight at 4 °C and then with the following primary antibodies in 2% milk in PBS-Tween buffer: anti-MuSK (1:1000, sc-33204, Santa Cruz Biotechnology), anti-YAP (1:1000, #4912, Cell Signaling Technology), antiYAPser127 (1:500, #4911, Cell Signaling Technology), anti-Alpha Dystroglycan (1:500, ab106110, Abcam) and anti-GAPDH (1:2000, #437000, Thermo Scientific). .. After washing, the membrane was incubated with PBS-Tween buffer containing HRP-conjugated goat anti-mouse and anti-rabbit IgG from Pierce (1:2000, PI-32230 (anti-mouse), PI-32260 (anti-rabbit)). ..

    Incubation:

    Article Title: Baccharis articulata aqueous extract exerts in vitro antifibrotic effect in hepatic stellate cells by attenuating collagen deposition and TGF-ß1 protein expression
    Article Snippet: After blocking, the individual primary horseradish peroxidase-conjugated antibodies (Santa Cruz Biotechnology, USA), PAI-1 (1:500 dilution), and TGF-β1 (1:500 dilution) were used to detect specific proteins. .. The membrane was subsequently washed thrice with PBS-Tween buffer, after which each blot was incubated with the corresponding secondary antibody for 1 h. Immunoreactive proteins were visualized by ECL detection using an iBright CL750 imaging system (Thermo Fisher Scientific, MA, USA). .. Expression levels were normalized against β-actin and quantified using the ImageJ software.

    Article Title: Proteomic and metabolomic profiling of aged pork loin chops reveals molecular phenotypes linked to pork tenderness
    Article Snippet: .. Incubation with a polyclonal rabbit anti-desmin antibody produced at Iowa State University ( ) diluted 1:40,000 in PBS-Tween buffer was conducted at 4 °C for ~18 h. After washing in PBS-Tween, the secondary goat anti-rabbit-HRP antibody (31460; Thermo Scientific, Waltham, MA, USA) was diluted 1:15,000 in PBS-Tween buffer, and membranes were incubated with the secondary antibody for 1 h at room temperature. .. After washing in PBS-Tween, immunoreactive desmin protein bands were detected using a chemiluminescent detection kit (ECL Prime; GE Healthcare, Piscataway, NJ, USA), and images of blots were obtained and analyzed using a ChemiImager 5500 (Alpha Innotech, San Leandro, CA, USA) and Alpha Ease FC software (version 3.03; Alpha Innotech).

    Article Title: CP12 controls ribulose 1,5 bisphosphate recycling and carbon acquisition in Chlamydomonas reinhardtii
    Article Snippet: After one hour of blocking (5% fat free milk in PBS-Tween20 0.1% buffer), the membrane was incubated overnight with primary antibodies against EPYC1 (Agrisera, diluted 1:5000) or against PRK (antibodies kindly provided by Dr Pierre Crozet ( Boisset et al ., 2023 ), diluted 1:10000). .. After washing with PBS-Tween buffer, the membrane was incubated for one hour with a horseradish peroxidase (HRP) coupled anti-rabbit secondary antibodies (Invitrogen, diluted 1:10000). ..

    Article Title: Deficiency of skeletal muscle Agrin contributes to the pathogenesis of age-related sarcopenia in mice
    Article Snippet: Samples (50 μg protein) were resolved by SDS‒PAGE and transferred to a nitrocellulose membrane, which was incubated in 5% milk in phosphate buffered saline (PBS)-0.3% Tween 20(#A600560, Sangon Biotech) overnight at 4 °C and then with the following primary antibodies in 2% milk in PBS-Tween buffer: anti-MuSK (1:1000, sc-33204, Santa Cruz Biotechnology), anti-YAP (1:1000, #4912, Cell Signaling Technology), anti-YAP ser127 (1:500, #4911, Cell Signaling Technology), anti-Alpha Dystroglycan (1:500, ab106110, Abcam) and anti-GAPDH (1:2000, #437000, Thermo Scientific). .. After washing, the membrane was incubated with PBS-Tween buffer containing HRP-conjugated goat anti-mouse and anti-rabbit IgG from Pierce (1:2000, PI-32230 (anti-mouse), PI-32260 (anti-rabbit)). ..

    Article Title: Deficiency of skeletal muscle Agrin contributes to the pathogenesis of age-related sarcopenia in mice.
    Article Snippet: Samples (50 μg protein) were resolved by SDS‒PAGE and transferred to a nitrocellulose membrane, which was incubated in 5% milk in phosphate buffered saline (PBS)-0.3% Tween 20(#A600560, Sangon Biotech) overnight at 4 °C and then with the following primary antibodies in 2% milk in PBS-Tween buffer: anti-MuSK (1:1000, sc-33204, Santa Cruz Biotechnology), anti-YAP (1:1000, #4912, Cell Signaling Technology), antiYAPser127 (1:500, #4911, Cell Signaling Technology), anti-Alpha Dystroglycan (1:500, ab106110, Abcam) and anti-GAPDH (1:2000, #437000, Thermo Scientific). .. After washing, the membrane was incubated with PBS-Tween buffer containing HRP-conjugated goat anti-mouse and anti-rabbit IgG from Pierce (1:2000, PI-32230 (anti-mouse), PI-32260 (anti-rabbit)). ..

    Imaging:

    Article Title: Baccharis articulata aqueous extract exerts in vitro antifibrotic effect in hepatic stellate cells by attenuating collagen deposition and TGF-ß1 protein expression
    Article Snippet: After blocking, the individual primary horseradish peroxidase-conjugated antibodies (Santa Cruz Biotechnology, USA), PAI-1 (1:500 dilution), and TGF-β1 (1:500 dilution) were used to detect specific proteins. .. The membrane was subsequently washed thrice with PBS-Tween buffer, after which each blot was incubated with the corresponding secondary antibody for 1 h. Immunoreactive proteins were visualized by ECL detection using an iBright CL750 imaging system (Thermo Fisher Scientific, MA, USA). .. Expression levels were normalized against β-actin and quantified using the ImageJ software.

    Produced:

    Article Title: Proteomic and metabolomic profiling of aged pork loin chops reveals molecular phenotypes linked to pork tenderness
    Article Snippet: .. Incubation with a polyclonal rabbit anti-desmin antibody produced at Iowa State University ( ) diluted 1:40,000 in PBS-Tween buffer was conducted at 4 °C for ~18 h. After washing in PBS-Tween, the secondary goat anti-rabbit-HRP antibody (31460; Thermo Scientific, Waltham, MA, USA) was diluted 1:15,000 in PBS-Tween buffer, and membranes were incubated with the secondary antibody for 1 h at room temperature. .. After washing in PBS-Tween, immunoreactive desmin protein bands were detected using a chemiluminescent detection kit (ECL Prime; GE Healthcare, Piscataway, NJ, USA), and images of blots were obtained and analyzed using a ChemiImager 5500 (Alpha Innotech, San Leandro, CA, USA) and Alpha Ease FC software (version 3.03; Alpha Innotech).

    Labeling:

    Article Title: 1-oxa-3,7-diazaspiro[4.5]decan-2-one derivatives as potent KRAS-G12D inhibitors: A multidisciplinary approach integrating machine learning, synthesis, and biological evaluation.
    Article Snippet: Oncogenic RAS mutations, which are common in human tumors and occur in about 30 % of cancer cases, present significant challenges to effective cancer treatment.. Among the KRAS family, the KRAS-G12D mutation is a promising target for treating different types of cancer.. Current approaches to inhibit the KRAS-G12D mutation have shown limited success, highlighting the urgent need for innovative therapies.

    Concentration Assay:

    Article Title: 1-oxa-3,7-diazaspiro[4.5]decan-2-one derivatives as potent KRAS-G12D inhibitors: A multidisciplinary approach integrating machine learning, synthesis, and biological evaluation.
    Article Snippet: Oncogenic RAS mutations, which are common in human tumors and occur in about 30 % of cancer cases, present significant challenges to effective cancer treatment.. Among the KRAS family, the KRAS-G12D mutation is a promising target for treating different types of cancer.. Current approaches to inhibit the KRAS-G12D mutation have shown limited success, highlighting the urgent need for innovative therapies.

    other:

    Article Title: Proteomic and metabolomic profiling of aged pork loin chops reveals molecular phenotypes linked to pork tenderness.
    Article Snippet: Incubation with a polyclonal rabbit anti-desmin antibody produced at Iowa State University (Huff-Lonergan et al., 1996b) diluted 1:40,000 in PBS-Tween buffer was conducted at 4 °C for ~18 h. After washing in PBS-Tween, the secondary goat anti-rabbit-HRP antibody (31460; Thermo Scientific, Waltham, MA, USA) was diluted 1:15,000 in PBSTween buffer, and membranes were incubated with the secondary antibody for 1 h at room temperature.



    Similar Products

    95
    Thermo Fisher superblocktm buffer
    Superblocktm Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+tween+buffer/Tween+20+blocking+buffer%2C+1%25+in+PBS/pm42271423-86-12-14
    Average 95 stars, based on 1 article reviews
    superblocktm buffer - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc tween 20 pbs t
    Tween 20 Pbs T, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+tween+buffer/Phosphate+Buffered+Saline+with+Tween+20/pmc12999298-65-8-30
    Average 96 stars, based on 1 article reviews
    tween 20 pbs t - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    95
    Thermo Fisher tween 20 pbst
    Tween 20 Pbst, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+tween+buffer/Tween+20+washing+buffer%2C+1%25+in+PBS/pm42237415-87-11-15
    Average 95 stars, based on 1 article reviews
    tween 20 pbst - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    94
    Thermo Fisher tween 20
    Tween 20, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+tween+buffer/BSA+Blocking+Buffer%2C+3%25+in+PBS%2C+with+0%2E05%25+Tween-20/pmc13208732-200-23-64
    Average 94 stars, based on 1 article reviews
    tween 20 - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    95
    Thermo Fisher pbs 10x
    Pbs 10x, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+tween+buffer/Tween+20+blocking+buffer%2C+1%25+in+PBS/bio_rxiv__64898__2026__05__20__726520-48-53-55
    Average 95 stars, based on 1 article reviews
    pbs 10x - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    Thermo Fisher pbs tween
    Pbs Tween, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+tween+buffer/Tween+20+washing+buffer%2C+1%25+in+PBS/pm42141523-381-19-23
    Average 95 stars, based on 1 article reviews
    pbs tween - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    Thermo Fisher tween 20 w v
    Tween 20 W V, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+tween+buffer/Tween+20+washing+buffer%2C+1%25+in+PBS/pm42125961-71-10-48
    Average 95 stars, based on 1 article reviews
    tween 20 w v - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    94
    Thermo Fisher v v tween 20 thermo fisher scientific 28360
    V V Tween 20 Thermo Fisher Scientific 28360, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+tween+buffer/Tween+20+washing+buffer%2C+0%2E5%25+in+PBS/bio_rxiv__64898__2026__05__11__724380-134-17-20
    Average 94 stars, based on 1 article reviews
    v v tween 20 thermo fisher scientific 28360 - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    95
    Thermo Fisher tween 20 in pbs
    Tween 20 In Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+tween+buffer/Tween+20+washing+buffer%2C+1%25+in+PBS/pm42115621-486-5-9
    Average 95 stars, based on 1 article reviews
    tween 20 in pbs - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    94
    Thermo Fisher v v tween 20 in pbs 1
    V V Tween 20 In Pbs 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+tween+buffer/Tween+20+washing+buffer%2C+0%2E5%25+in+PBS/bio_rxiv__64898__2026__05__05__722894-40-20-8
    Average 94 stars, based on 1 article reviews
    v v tween 20 in pbs 1 - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    Image Search Results